High-efficiency detection of severe acute respiratory syndrome virus genetic material
Identifieur interne : 005B33 ( Main/Exploration ); précédent : 005B32; suivant : 005B34High-efficiency detection of severe acute respiratory syndrome virus genetic material
Auteurs : Manfred Weidmann [Allemagne] ; Paolo M. D. A. Zanotto [Allemagne] ; Friedemann Weber [Allemagne] ; Martin Spiegel [Allemagne] ; Hans Rheinhard Brodt [Brésil] ; Frank T. Hufert [Allemagne]Source :
- Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ARN viral.
- génétique : Virus du SRAS.
- virologie : Liquide de lavage bronchoalvéolaire.
- Pascal (Inist)
- Wicri :
- topic : Génétique.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : RNA, Viral.
- genetics : SARS Virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Bronchoalveolar Lavage Fluid.
- Humans, Sensitivity and Specificity.
Abstract
A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log10 times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log10 times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.
Url:
Affiliations:
- Allemagne, Brésil
- Bade-Wurtemberg, District de Darmstadt, District de Fribourg-en-Brisgau, Hesse (Land), État de São Paulo
- Francfort-sur-le-Main, Fribourg-en-Brisgau, São Paulo
- Université de São Paulo
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Le document en format XML
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D. A." last="Zanotto">Paolo M. D. A. Zanotto</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Zentrum der Inneren Medizin, Medizinische Klinik III/Infektiologie</s1><s2>60590 Frankfurt am Main</s2><s3>DEU</s3><sZ>2 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Darmstadt</region><settlement type="city">Francfort-sur-le-Main</settlement></placeName></affiliation></author><author><name sortKey="Weber, Friedemann" sort="Weber, Friedemann" uniqKey="Weber F" first="Friedemann" last="Weber">Friedemann Weber</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Virology, Institute of Medical Microbiology and Hygiene, University of Freiburg</s1><s2>79008 Freiburg</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Fribourg-en-Brisgau</region><settlement type="city">Fribourg-en-Brisgau</settlement></placeName></affiliation></author><author><name sortKey="Spiegel, Martin" sort="Spiegel, Martin" uniqKey="Spiegel M" first="Martin" last="Spiegel">Martin Spiegel</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Virology, Institute of Medical Microbiology and Hygiene, University of Freiburg</s1><s2>79008 Freiburg</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Fribourg-en-Brisgau</region><settlement type="city">Fribourg-en-Brisgau</settlement></placeName></affiliation></author><author><name sortKey="Brodt, Hans Rheinhard" sort="Brodt, Hans Rheinhard" uniqKey="Brodt H" first="Hans Rheinhard" last="Brodt">Hans Rheinhard Brodt</name><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Departamento de Microbiologia, Instituto de Ciências Biomédicas Universidade de São Paulo</s1><s2>05508-000, São Paulo</s2><s3>BRA</s3><sZ>5 aut.</sZ></inist:fA14><country>Brésil</country><placeName><settlement type="city">São Paulo</settlement><region type="state">État de São Paulo</region></placeName><orgName type="university">Université de São Paulo</orgName></affiliation></author><author><name sortKey="Hufert, Frank T" sort="Hufert, Frank T" uniqKey="Hufert F" first="Frank T." last="Hufert">Frank T. Hufert</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Virology, Institute of Medical Microbiology and Hygiene, University of Freiburg</s1><s2>79008 Freiburg</s2><s3>DEU</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Fribourg-en-Brisgau</region><settlement type="city">Fribourg-en-Brisgau</settlement></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of clinical microbiology : (Print)</title><title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title><idno type="ISSN">0095-1137</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of clinical microbiology : (Print)</title><title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title><idno type="ISSN">0095-1137</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bronchoalveolar Lavage Fluid (virology)</term><term>Detection</term><term>Genetics</term><term>Humans</term><term>Microbiology</term><term>RNA, Viral (analysis)</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>SARS Virus (genetics)</term><term>Sensitivity and Specificity</term><term>Severe acute respiratory syndrome</term><term>Virus</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term><term>Humains</term><term>Liquide de lavage bronchoalvéolaire (virologie)</term><term>RT-PCR ()</term><term>Sensibilité et spécificité</term><term>Virus du SRAS (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Liquide de lavage bronchoalvéolaire</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Bronchoalveolar Lavage Fluid</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Humains</term><term>RT-PCR</term><term>Sensibilité et spécificité</term><term>Virus</term><term>Détection</term><term>Génétique</term><term>Microbiologie</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log<sub>10</sub> times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log<sub>10</sub> times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Brésil</li></country><region><li>Bade-Wurtemberg</li><li>District de Darmstadt</li><li>District de Fribourg-en-Brisgau</li><li>Hesse (Land)</li><li>État de São Paulo</li></region><settlement><li>Francfort-sur-le-Main</li><li>Fribourg-en-Brisgau</li><li>São Paulo</li></settlement><orgName><li>Université de São Paulo</li></orgName></list><tree><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Weidmann, Manfred" sort="Weidmann, Manfred" uniqKey="Weidmann M" first="Manfred" last="Weidmann">Manfred Weidmann</name></region><name sortKey="Hufert, Frank T" sort="Hufert, Frank T" uniqKey="Hufert F" first="Frank T." last="Hufert">Frank T. Hufert</name><name sortKey="Spiegel, Martin" sort="Spiegel, Martin" uniqKey="Spiegel M" first="Martin" last="Spiegel">Martin Spiegel</name><name sortKey="Weber, Friedemann" sort="Weber, Friedemann" uniqKey="Weber F" first="Friedemann" last="Weber">Friedemann Weber</name><name sortKey="Zanotto, Paolo M D A" sort="Zanotto, Paolo M D A" uniqKey="Zanotto P" first="Paolo M. D. A." last="Zanotto">Paolo M. D. A. Zanotto</name></country><country name="Brésil"><region name="État de São Paulo"><name sortKey="Brodt, Hans Rheinhard" sort="Brodt, Hans Rheinhard" uniqKey="Brodt H" first="Hans Rheinhard" last="Brodt">Hans Rheinhard Brodt</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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